English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/4098
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 25 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Characterization of two highly similar Rad51 homologs of Physcomitrella patens
Autor : Ayora, Silvia; Piruat, José I.; Luna, Rosa ; Reiss, Bernd; Russo, Vincenzo E. A.; Aguilera, Andrés ; Alonso, Juan Carlos
Palabras clave : DNA recombination
DNA repair
RecA
ATPases
Strand exchange
Fecha de publicación : 8-feb-2002
Editor: Elsevier
Citación : J. Mol. Biol. 316, 35-49 (2002).
http://dx.doi.org/10.1006/jmbi.2001.5336
Resumen: The moss Physcomitrella patens, which is a land plant with efficient homologous recombination, encodes two Rad51 proteins (PpaRad51.1 and PpaRad51.2). The PpaRad51.1 and PpaRad51.2 proteins, which share 94 % identity between them, interact with themselves and with each other. Both proteins bind ssDNA and dsDNA in a Mg(2+) and pH-dependent manner, with a stoichiometry of one PpaRad51.1 monomer per 3(+/-1) nt or bp and one PpaRad51.2 monomer per 1(+/-0.5) nt or bp, respectively. At neutral pH, a 1.6-fold excess of both proteins is required for ssDNA and dsDNA binding. PpaRad51.1 and PpaRad51.2 show ssDNA-dependent ATPase activity and efficiently promote strand annealing in a nucleotide-independent but in a Mg(2+)-dependent manner. Both proteins promote joint-molecule formation, DNA strand invasion and are able to catalyse strand exchange in the presence of Mg(2+) and ATP. No further increase in the activities is observed when both proteins are present in the same reaction. None of the PpaRad51 gene products complement the DNA repair and recombination phenotype of Saccharomyces cerevisiae rad51delta mutants. However, PpaRad51.1 confers a dominant-negative DNA repair phenotype, and both PpaRad51 proteins reduce the levels of double-strand break-induced recombination when overexpressed in S. cerevisiae wt cells. These results suggest that both PpaRad51 proteins are bona fide Rad51 proteins that may contribute, in a different manner, to homologous recombination, and that they might replace ScRad51 in a hypothetical yeast protein complex inactivating different functions required for recombinational repair.
Descripción : PMID: 11829501.-- Final full-text version of the paper available at: http://www.sciencedirect.com/science/journal/00222836
URI : http://hdl.handle.net/10261/4098
DOI: 10.1006/jmbi.2001.5336
ISSN: 0022-2836
Aparece en las colecciones: (CNB) Artículos
(CABIMER) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.