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dc.contributor.author | Cortés-Ledesma, Felipe | - |
dc.contributor.author | Tous, Cristina | - |
dc.contributor.author | Aguilera, Andrés | - |
dc.date.accessioned | 2008-05-07T17:07:40Z | - |
dc.date.available | 2008-05-07T17:07:40Z | - |
dc.date.issued | 2007-10 | - |
dc.identifier.citation | Nucleic Acids Res. 35(19): 6560–6570 (2007). | en_US |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10261/4079 | - |
dc.description | PMCID: PMC2095809 | en_US |
dc.description.abstract | Homologous recombination (HR) is the major mechanism used to repair double-strand breaks (DSBs) that result from replication, but a study of repair of DSBs specifically induced during S-phase is lacking. Using an inverted-repeat assay in which a DSB is generated by the encountering of the replication fork with nicks, we can physically detect repair by sister-chromatid recombination (SCR) and intra-chromatid break-induced replication (IC-BIR). As expected, both events depend on Rad52, but, in contrast to previous data, both require Rad59, suggesting a prominent role of Rad59 in repair of replication-born DSBs. In the absence of Rad51, SCR is severely affected while IC-BIR increases, a phenotype that is also observed in the absence of Rad54 but not of its paralog Rdh54/Tid1. These data are consistent with SCR occurring by Rad51-dependent mechanisms assisted by Rad54, and indicate that in the absence of strand exchange-dependent SCR, breaks can be channeled to IC-BIR, which works efficiently in the absence of Rad51. Our study provides molecular evidence for inversions between repeats occurring by BIR followed by single-strand annealing (SSA) in the absence of strand exchange. | en_US |
dc.description.sponsorship | This work was supported by grants from the Ministry of Science of Education of Spain (SAF2003-00204 and BFU2006-05260) and Junta de Andalucía (CVI102 and CVI624). F.C.-L. was the recipient of a pre-doctoral training grant from the Spanish Ministry of Health. Funding to pay the Open Access publication charges for this article was provided by the Spanish Ministry of Science and Education (grant BFU2006-05260). | en_US |
dc.format.extent | 22185 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Oxford University Press | en_US |
dc.rights | openAccess | en_US |
dc.subject | Homologous recombination | en_US |
dc.subject | DNA double-strand breaks | en_US |
dc.subject | DNA repair | en_US |
dc.title | Different genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replication | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1093/nar/gkm488 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.identifier.pmid | 17905819 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.languageiso639-1 | en | - |
item.grantfulltext | open | - |
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NuclAcRes_200710.pdf | 5,87 MB | Adobe PDF | Visualizar/Abrir |
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