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An efficient regeneration system via somatic embryogenesis in olive

AuthorsCerezo, Sergio; Mercado, José A.; Pliego-Alfaro, Fernando
KeywordsIn vitro plant regeneration
Olea europaea
Semi-permeable membrane
Somatic embryo
Suspension culture
Water potential
Issue DateAug-2011
CitationPlant Cell, Tissue and Organ Culture
AbstractOlive is one of the most important oil crops in the Mediterranean area. Biotechnological improvement of this species is hampered by the recalcitrant nature of olive tissue regeneration in vitro. In this investigation, we have developed an efficient regeneration system for juvenile olive explants via somatic embryogenesis. Embryogenic cultures were obtained at a rate of 25% by culturing isolated radicles from mature seeds in a modified olive medium (OMc) containing 2.5 μM 6-(dimethylallylamino) purine (2iP) and 25 μM indole-3-butyric acid (IBA) over 3 weeks and later transferring to the same medium without 2iP and with a lower IBA concentration. Two different basal formulations, OMc and olive cyclic embryogenesis medium (ECO) [1/4 OM macroelements, 1/4 Murashige and Skoog (MS) microelements and 1/2 OM vitamins supplemented with 550 mg l-1 glutamine], were tested for embryogenic callus proliferation and maturation.
Description(The original publication is available at www.springerlink.com)
Publisher version (URL)http://www.springerlink.com/content/768106x5k5230r33/
Appears in Collections:(IHSM) Artículos
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