English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/4071
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Genetic dissection of a stem cell niche: the case of the Drosophila ovary

AuthorsBolívar, Jorge; Pearson, John ; López-Onieva, Lourdes ; González-Reyes, Acaimo
KeywordsDrosophila oogenesis
Ovarian stem cell niche
Genetic analysis
Issue DateNov-2006
PublisherJohn Wiley & Sons
CitationDevelopmental Dynamics 235(11): 2969-2979 (2006)
AbstractIn this work, we demonstrate a powerful new tool for the manipulation of the stromal component of a well-established Drosophila stem cell niche. We have generated a bric-a-brac 1 (bab1)-Gal4 line that drives UAS expression in many somatic ovary cell types from early larval stages. Using this Gal4 line, we could effectively induce FLP/FRT-mediated recombination in the stromal cells of the ovarian germline stem cell niche. Mutant clones were observed in the developing ovary of larvae and pupae, including in somatic cell types that do not divide in the adult, such as the cap cells and the terminal filament cells. Exploiting the ability of bab1-Gal4 to generate large clones, we demonstrate that bab1-Gal4 is an effective tool for analyzing stem cell niche morphogenesis and cyst formation in the germarium. We have identified a novel requirement for engrailed in the correct organization of the terminal filaments. We also demonstrate an involvement for integrins in cyst formation and follicle cell encapsulation. Finally using bab1-Gal4 in conjunction with the Gal80 system, we show that while ectopic dpp expression from stromal cells is sufficient to induce hyperplastic stem cell growth, neither activation nor inactivation of the BMP pathway within stromal cells affects germline stem cell maintenance.
Description11 páginas, 6 figuras, 2 tablas.-- El material suplementario referido en este artículo puede verse en www.interscience.wiley.com/jpages/1058-8388/suppmat
Publisher version (URL)http://dx.doi.org/10.1002/dvdy.20967
URIhttp://hdl.handle.net/10261/4071
DOI10.1002/dvdy.20967
ISSN1058-8388
Appears in Collections:(CABD) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.