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Title

An efficient approach to isolate STAT regulated enhancers uncovers STAT92E fundamental role in Drosophila tracheal development

AuthorsSotillos, Sol ; Espinosa-Vázquez, José Manuel; Foglia, Filippo ; Hu, Nan; Castelli-Gair Hombría, James
KeywordsSTAT gene-regulation
Trachea specification
Ventral veinless
Trachealess
Enhacer localization
Gene-desert
Drosophila
Immunohistochemistry
Issue Date18-Feb-2010
PublisherElsevier
CitationDevelopmental Biology 340(2): 571-582 (2010)
AbstractThe ventral veinless (vvl) and trachealess (trh) genes are determinants of the Drosophila trachea. Early in development both genes are independently activated in the tracheal primordia by signals that are ill defined. Mutants blocking JAK/STAT signaling at any level do not form a tracheal tree suggesting that STAT92E may be an upstream transcriptional activator of the early trachea determinants. To test this hypothesis we have searched for STAT92E responsive enhancers activating the expression of vvl and trh in the tracheal primordia. We show that STAT92E regulated enhancers can be rapidly and efficiently isolated by focusing the analysis on genomic regions with clusters of putative STAT binding sites where at least some of them are phylogenetically conserved. Detailed analysis of a vvl early tracheal enhancer shows that non-conserved sites collaborate with conserved sites for enhancer activation. We find that STAT92E regulated enhancers can be located as far 60 kb from the promoters. Our results indicate that vvl and trh are independently activated by STAT92E which is the most important transcription factor required for trachea specification.
Description12 páginas, 7 figuras.-- Under a Creative Commons license.
Publisher version (URL)http://dx.doi.org/10.1016/j.ydbio.2010.02.015
URIhttp://hdl.handle.net/10261/40665
DOI10.1016/j.ydbio.2010.02.015
ISSN0012-1606
Appears in Collections:(CABD) Artículos
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