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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/40518
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dc.contributor.authorSantana, Margarida-
dc.contributor.authorPortillo Guisado, María del Carmen-
dc.contributor.authorGonzález Grau, Juan Miguel-
dc.date.accessioned2011-10-04T09:41:04Z-
dc.date.available2011-10-04T09:41:04Z-
dc.date.issued2010-08-
dc.identifier.citationJournal of Biotechnology 149 (1-2): 21-23 (2010)es_ES
dc.identifier.issn0168-1656-
dc.identifier.urihttp://hdl.handle.net/10261/40518-
dc.description3 pages, 1 figure, 1 table, 4 references.es_ES
dc.description.abstractRecombinant gene strategies using fusion tags for purification are essential procedures to obtain large protein quantities. However, most cloning systems result in recombinant proteins with added amino acids inexistent in their native forms which can lead to significant changes in protein properties. An original and simple cloning strategy is proposed to obtain proteins identical in amino acid sequence to the native proteins.es_ES
dc.description.sponsorshipThe authors acknowledge funding from the Government of Andalusia (Spain) BIO288. M. Santana was recipient of grant SFRH/BPD/34404/2006 from the Fundação para a Ciência e a Tecnologia (Portugal).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsopenAccesses_ES
dc.subjectCloning strategyes_ES
dc.subjectExpression vectorses_ES
dc.subjectNative protein; Recombinant proteines_ES
dc.subjectFusion tages_ES
dc.subjectBiocatalystses_ES
dc.titleA cloning strategy to obtain recombinant proteins with identical primary structure to the native formses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.jbiotec.2010.06.010-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/doi:10.1016/j.jbiotec.2010.06.010es_ES
dc.contributor.funderJunta de Andalucía-
dc.contributor.funderFundação para a Ciência e a Tecnologia (Portugal)-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001871es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100011011es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.openairetypeartículo-
item.grantfulltextopen-
item.languageiso639-1en-
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