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A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms

AuthorsSantana, Margarida; Portillo Guisado, María del Carmen CSIC ORCID; González Grau, Juan Miguel CSIC ORCID
KeywordsCloning strategy
Expression vectors
Native protein; Recombinant protein
Fusion tag
Issue DateAug-2010
CitationJournal of Biotechnology 149 (1-2): 21-23 (2010)
AbstractRecombinant gene strategies using fusion tags for purification are essential procedures to obtain large protein quantities. However, most cloning systems result in recombinant proteins with added amino acids inexistent in their native forms which can lead to significant changes in protein properties. An original and simple cloning strategy is proposed to obtain proteins identical in amino acid sequence to the native proteins.
Description3 pages, 1 figure, 1 table, 4 references.
Publisher version (URL)http://dx.doi.org/doi:10.1016/j.jbiotec.2010.06.010
Appears in Collections:(IRNAS) Artículos
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