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| Título: | A cloning strategy to obtain recombinant proteins with identical primary structure to the native forms |
Autor: | Santana, Margarida; Portillo Guisado, María del Carmen CSIC ORCID; González Grau, Juan Miguel CSIC ORCID | Palabras clave: | Cloning strategy Expression vectors Native protein; Recombinant protein Fusion tag Biocatalysts |
Fecha de publicación: | ago-2010 | Editor: | Elsevier | Citación: | Journal of Biotechnology 149 (1-2): 21-23 (2010) | Resumen: | Recombinant gene strategies using fusion tags for purification are essential procedures to obtain large protein quantities. However, most cloning systems result in recombinant proteins with added amino acids inexistent in their native forms which can lead to significant changes in protein properties. An original and simple cloning strategy is proposed to obtain proteins identical in amino acid sequence to the native proteins. | Descripción: | 3 pages, 1 figure, 1 table, 4 references. | Versión del editor: | http://dx.doi.org/doi:10.1016/j.jbiotec.2010.06.010 | URI: | http://hdl.handle.net/10261/40518 | DOI: | 10.1016/j.jbiotec.2010.06.010 | ISSN: | 0168-1656 |
| Aparece en las colecciones: | (IRNAS) Artículos |
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| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| A clonning strategy to obtain recombinant proteins.pdf | 106,41 kB | Adobe PDF |  Visualizar/Abrir |
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