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Título

The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis

Autor Abdu, Uri; González-Reyes, Acaimo ; Ghabrial, Amin; Schüpbach, Trudi
Palabras clave Mutations
Drosophila
DNA breaks
Repair enzymes
Recombination
Meiosis
Fecha de publicación sep-2003
EditorGenetics Society of America
Citación Genetics 165: 197–204 (September 2003).
http://www.genetics.org/cgi/content/abstract/165/1/197
ResumenIn Drosophila, mutations in double-strand DNA break (DSB) repair enzymes, such as spn-B, activate a meiotic checkpoint leading to dorsal-ventral patterning defects in the egg and an abnormal appearance of the oocyte nucleus. Mutations in spn-D cause an array of ovarian phenotypes similar to spn-B. We have cloned the spn-D locus and found that it encodes a protein of 271 amino acids that shows significant homology to the human RAD51C protein. In mammals the spn-B and spn-D homologs, XRCC3 and RAD51C, play a role in genomic stability in somatic cells. To test for a similar role for spn-B and spn-D in double-strand DNA repair in mitotic cells, we analyzed the sensitivity of single and double mutants to DSBs induced by exposure to X rays and MMS. We found that neither singly mutant nor doubly mutant animals were significantly sensitized to MMS or X rays. These results suggest that spn-B and spn-D act in meiotic recombination but not in repair of DSBs in somatic cells. As there is no apparent ortholog of the meiosis-specific DMC1 gene in the Drosophila genome, and given their meiosis-specific requirement, we suggest that spn-B and spn-D may have a function comparable to DMC1.
Descripción Sequence data from this article have been deposited with the Gen-Bank data libraries under accession no. AY257540.
URI http://hdl.handle.net/10261/4048
ISSN0016-6731
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