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Title

New application of air-dry techniques for studying Ephemeroptera and Plecoptera eggs by Scanning Electron Microscopy

AuthorsUbero-Pascal, N.; Fortuño Alós, José Manuel ; Puig, Mariàngels
KeywordsSEM
Hexamethyl-disilizane
Egg morphology
Air-drying techniques
Critical point drying
Tetramethylsilane
Issue Date2005
PublisherWiley-Blackwell
CitationMicroscopy Research and Technique 68 : 264-271 (2005)
AbstractHexamethyldisilizane (HMDS) and tetramethylsilane are organic compounds that are volatile at ambient temperature and which can therefore be used for air-drying biological samples for SEM studies. The techniques using these compounds provide results that are comparable with those obtained by critical point drying, but which involve a very simple process that saves time and money. Both techniques were applied to SEM studies of Ephemeroptera and Plecoptera eggs in order to assess their suitability as alternative methods to critical point drying for these kinds of biological material. The results show no morphological differences between eggs HMDS air-dried and critical point-dried.
Description8 páginas, 6 figuras.
Publisher version (URL)http://dx.doi.org/10.1002/jemt.20248
URIhttp://hdl.handle.net/10261/40443
DOI10.1002/jemt.20248
ISSN1059-910X
Appears in Collections:(CEAB) Artículos
(ICM) Artículos
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