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A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation

AuthorsRoyo, José Luis ; Manyani, Hamid; Cebolla, Ángel; Santero, Eduardo
KeywordsBacterial proteins
DNA-binding proteins
Gene expression regulation
Klebsiella oxytoca
Pseudomonas putida
RNA- binding
Transcription factors
XylS protein
Pseudomonas putida
Antiterminator proteins
Issue Date31-Oct-2005
PublisherOxford University Press
CitationNucleic Acids Research 33(19): e169 (2005)
AbstractA major drawback of regulated gene expression from vectors bearing strong promoters is the associated high basal expression level. Simple regulatory systems have an intrinsic limitation in the range of induction, and attempts to mutate promoters to reduce basal expression usually result in concomitant reduction of induced levels. We have explored the possibility of reducing basal levels of gene expression while keeping induced levels intact by incorporating an additional regulatory circuit controlling a different step of the expression process. We have integrated the nasFEDCBA transcriptional attenuation system of Klebsiella oxytoca into a cascade expression circuit based on different regulatory elements of Pseudomonas putida, and also into a system based on the tac promoter, to expand their regulatory capacity. Basal expression from the promoters of these circuits was reduced by more than 10-fold by the nasF attenuator sequence while keeping the induced levels intact in the presence of the antiterminator protein, thus increasing the induction ratio by up to 1700-fold. In addition, using different combinations of regulatory elements and inducing conditions, we were able to obtain a broad range of expression levels. These vectors and the concept of their design will be very useful in regulating overproduction of heterologous proteins both at laboratory and industrial scales.
Description10 páginas, 3 figuras, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1093/nar/gni168
Appears in Collections:(CABD) Artículos
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