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dc.contributor.authorMatías-Román, Salomón-
dc.contributor.authorGálvez, Beatriz G.-
dc.contributor.authorGenís, Laura-
dc.contributor.authorYáñez-Mó, María-
dc.contributor.authorRosa, Gonzalo de la-
dc.contributor.authorSánchez-Mateos, Paloma-
dc.contributor.authorSánchez-Madrid, Francisco-
dc.contributor.authorArroyo, Alicia G.-
dc.identifier.citationBlood, 105: 3956-3964 (2005)es_ES
dc.description7 Figureses_ES
dc.description.abstractMembrane type 1–matrix metalloproteinase (MT1-MMP) is involved in endothelial and tumor-cell migration, but its putative role in leukocyte migration has not been characterized yet. Here, we demonstrate that anti–MT1-MMP monoclonal antibody (mAb) impaired monocyte chemotactic protein-1 (MCP-1)–stimulated monocyte migration on fibronectin (FN), vascular cell adhesion molecule-1 (VCAM-1), and intercellular adhesion molecule-1 (ICAM-1). In addition, monocyte transmigration through tumor necrosis factor-α (TNF-α)–activated endothelium is also inhibited by anti–MT1-MMP mAb. Therefore, regulation of MT1-MMP in human peripheral blood monocytes was investigated. First, MT1-MMP clustering was observed at motility-associated membrane protrusions of MCP-1–stimulated monocytes migrating on FN, VCAM-1, or ICAM-1 and at the leading edge, together with profilin, of monocytes transmigrating through activated endothelial cells. In addition, up-regulation of MT1-MMP expression was induced in human monocytes upon attachment to FN in a manner dependent on α41 and α51 integrins. Binding of monocytes to TNF-α–activated human endothelial cells as well as to VCAM-1 or ICAM-1 also resulted in an increase of MT1-MMP expression. These findings correlated with an enhancement of MT1-MMP fibrinolytic activity in monocytes bound to FN, VCAM-1, or ICAM-1. Our data show that MT1-MMP is required during human monocyte migration and endothelial transmigration and that MT1-MMP localization, expression, and activity are regulated in monocytes upon contact with FN or endothelial ligands, pointing to a key role of MT1-MMP in monocyte recruitment during inflammation.es_ES
dc.description.sponsorshipCentro Nacional de Investigaciones Cardiovasculares, Madrid,Spain; Departamento de Inmunología, Hospital Universitario de la Princesa,Madrid, Spain; and the Departamento de Inmunología, Hospital Universitario Gregorio Maranón, Madrid, Spain.es_ES
dc.publisherAmerican Society of Hematologyes_ES
dc.subjectMembrane type 1–matrix metalloproteinase (MT1-MMP)es_ES
dc.subjecttumor-cell migrationes_ES
dc.titleMembrane type 1–matrix metalloproteinase is involved in migration of human monocytes and is regulated through their interaction with fibronectin or endotheliumes_ES
dc.description.peerreviewedPeer reviewedes_ES
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