Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/40198
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Agouti protein, mahogunin, and attractin in pheomelanogenesis and melanoblast-like alteration of melanocytes: a cAMP-independent pathway

AuthorsHida, Tokimasa; Wakamatsu, Kazumasa; Sviderskaya, Elena V.; Donkin, Andrew J.; Montoliu, Lluís CSIC ORCID ; Lamoreux, M. Lynn; Yu, Bin; Millhauser, Glenn L.; Ito, Shosuke; Barsh, Gregory S.; Jimbow, Kowichi; Bennett, Dorothy C.
KeywordsPheomelanin
Mc1r
Melanoblast
Attractin
Mahogunin
Agouti
Issue Date15-May-2009
PublisherJohn Wiley & Sons
CitationPigment Cell and Melanoma Research 22(5): 623–634 (2009)
AbstractMelanocortin-1 receptor (MC1R) and its ligands, a-melanocyte stimulating hormone (aMSH) and agouti signaling protein (ASIP), regulate switching between eumelanin and pheomelanin synthesis in melanocytes. Here we investigated biological effects and signaling pathways of ASIP. Melan-a non agouti (a ⁄ a) mouse melanocytes produce mainly eumelanin, but ASIP combined with phenylthiourea and extra cysteine could induce over 200-fold increases in the pheomelanin to eumelanin ratio, and a tan-yellow color in pelletted cells.
Moreover, ASIP-treated cells showed reduced proliferation and a melanoblast-like appearance, seen also in melanocyte lines from yellow (Ay ⁄ a and Mc1re ⁄ Mc1re) mice. However ASIP-YY, a C-terminal fragment of ASIP, induced neither biological nor pigmentary changes. As, like ASIP, ASIP-YY inhibited the cAMP rise induced by aMSH analog NDP-MSH, and reduced cAMP level without added MSH, the morphological changes and depigmentation seemed independent of cAMP signaling. Melanocytes genetically null for ASIP mediators attractin or mahogunin (Atrnmg-3J ⁄ mg-3J or Mgrn1md-nc ⁄ md-nc) also responded to both ASIP and ASIP-YY in cAMP level, while only ASIP altered their proliferation and (in part) shape. Thus, ASIP–MC1R signaling includes a cAMP-independent pathway through attractin and mahogunin, while the known cAMP-dependent component requires neither attractin nor mahogunin.
Publisher version (URL)http://dx.doi.org/10.1111/j.1755-148X.2009.00582.x
URIhttp://hdl.handle.net/10261/40198
DOI10.1111/j.1755-148X.2009.00582.x
ISSN1755-1471
E-ISSN1755-148X
Appears in Collections:(CNB) Artículos

Show full item record
Review this work

PubMed Central
Citations

26
checked on Jan 14, 2022

SCOPUSTM   
Citations

59
checked on Jan 12, 2022

WEB OF SCIENCETM
Citations

48
checked on Jan 18, 2022

Page view(s)

385
checked on Jan 19, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.