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Title

Molecular S-RNase genotyping and determination of cross-incompatibility groups in loquat [Eriobotrya japonica (Thunb.) Lindl.]

AuthorsCarrera García, Laura ; Sanzol Sanz, Javier; Soler, Esteve; Herrero Romero, María ; Hormaza Urroz, José Ignacio
KeywordsS-RNase
loquat
Eriobotrya japonica (Thunb.) Lindl
Issue DateSep-2011
PublisherSpringer
CitationCarrera L, Sanzol J, Soler E, Herrero M, Hormaza JI. Molecular S-RNase genotyping and determination of cross-incompatibility groups in loquat [Eriobotrya japonica (Thunb.) Lindl.]. Euphytica 181 (2): 267-275 (2011)
SeriesHormaza
20
AbstractLoquat (Eriobotrya japonica Lindl.; Pyrinae, Rosaceae) is an evergreen fruit tree which is acquiring increasing importance as an alternative crop in warm-temperate and subtropical regions. Recent pollination studies suggest that crop intensification has resulted in a reduction of fruit productivity in different locations. Loquat exhibits gametophytic self-incompatibility (GSI) and, consequently, fruit set strongly depends on cross-pollination between intercompatible cultivars. S-genotyping and incompatibility group assignment is therefore essential for adequate loquat orchard design. This study reports the molecular characterization of the S-RNase allele composition of 31 loquat cultivars grown in Spain. PCR with primers developed from loquat S-RNase sequences was used in combination with high-performance capillary polyacrilamide electrophoresis for the fine-evaluation of length polymorphisms at the S-RNase gene. This approach allowed to uncover a new S-RNase allele (S5), whose sequence is reported and to characterize the first six cross-incompatibility groups (I-VI) in loquat, resulting from the combination of five S-alleles (S1 to S5)
Publisher version (URL)http://www.springerlink.com/content/q289q88g2g287g0j/
URIhttp://hdl.handle.net/10261/40120
DOI10.1007/s10681-011-0426-x
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