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Title

On-capillary derivatization and analysis of amino acids in human plasma by capillary electrophoresis with laser-induced fluorescence detection: Application to diagnosis of aminoacidopathies

AuthorsVeledo, Maria Teresa; Frutos, Mercedes de; Diez-Masa, Jose Carlos
KeywordsAminoacidopathies
Amino acids
Capillary electrophoresis
Laser-induced fluorescence
Issue Date2006
PublisherJohn Wiley & Sons
CitationElectrophoresis 27 (15) : 3101–3107 (2006)
AbstractA capillary electrophoresis with laser-induced fluorescence detection method for the analysis of free amino acids (AA) in human plasma was developed. A mixture of 16 AA was on-capillary derivatized with 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ) and separated inside the capillary in less than 30 min using 70 mM borax-3.5 mM SDS pH 9.3 as running buffer. Four plasma samples from a healthy donor and patients suf- fering from phenylketonuria, propionic acidemia, and tyrosinemia type II were studied. Repeatabilities calculated as intra-day RSD (n = 3) values for the AA involved in these aminoacidopathies (glycine, phenylalanine, and tyrosine) were in the range of 0.3 to 1.2% for migration time and 3.7 to 8.2% for peak height. Reproducibilities calculated as inter-day RSD (n = 4) values for the same AA were between 0.7 and 1.4% for migration time and 4.7 and 9.1% for peak height. A fast qualitative analysis allowed the identification of the corresponding disease by comparing the electrophoretic profiles from the patient and the healthy donor and noting the increased level of the specific AA accompanying each individual disease. The results of the quantitative analysis for glycine, phenylalanine, and tyrosine in the plasma samples studied using the devel- oped method showed a good agreement with those provided by the Center of Diag- nosis of Molecular Diseases using a standard method for AA analysis.
Publisher version (URL)http://dx.doi.org/10.1002/elps.200500781
URIhttp://hdl.handle.net/10261/40023
DOI10.1002/elps.200500781
ISSN0173-0835
Appears in Collections:(IQOG) Artículos
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