Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/39738
COMPARTIR / EXPORTAR:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

In vitro evolution of terminal protein-containing genomes

AutorEsteban, José A. CSIC ORCID; Blanco, Luis CSIC ORCID ; Villar, Laurentino CSIC; Salas, Margarita CSIC ORCID
Palabras claveφ29 DNA replication
DNA amplification
Fecha de publicación1-abr-1997
EditorNational Academy of Sciences (U.S.)
CitaciónProceedings of the National Academy of Sciences of the USA 94(7): 2921-2926 (1997)
ResumenA new self-sustained terminal protein-primed DNA amplification system has been used to describe in vitro evolutionary changes affecting maintenance of the genome size of bacteriophage φ29. These changes involve generation and efficient amplification of short palindromic molecules containing an inverted duplication of one of the original DNA ends. A template-switching mechanism is proposed to account for the appearance of these molecules. After their formation, they would replicate by means of hairpin intermediates. Relevant kinetic information about this DNA replication system has been obtained from the competition between the input full-length φ29 DNA and its derived truncated versions. The physiological relevance of these molecules and the mechanisms to control their formation are discussed.
Versión del editorhttp://www.pnas.org/content/94/7/2921.full.pdf
URIhttp://hdl.handle.net/10261/39738
ISSN0027-8424
E-ISSN1091-6490
Aparece en las colecciones: (CBM) Artículos




Ficheros en este ítem:
Fichero Descripción Tamaño Formato
PNAS-1997-Esteban-2921-6.pdf332,67 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo

CORE Recommender

Page view(s)

296
checked on 27-mar-2024

Download(s)

153
checked on 27-mar-2024

Google ScholarTM

Check


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.