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dc.contributor.authorMartín, Carolina-
dc.contributor.authorHerrero Romero, María-
dc.contributor.authorHormaza Urroz, José Ignacio-
dc.date.accessioned2011-09-19T08:02:09Z-
dc.date.available2011-09-19T08:02:09Z-
dc.date.issued2011-08-
dc.identifier.citationMartín C, Herrero M, Hormaza JI. Molecular Characterization of Apricot Germplasm from an Old Stone Collection. Plos One 6 (8): e23979 (2011)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/39634-
dc.description9 Pag., 3 Tabl., 2 Fig. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es_ES
dc.description.abstractIncreasing germplasm erosion requires the recovery and conservation of traditional cultivars before they disappear. Here we present a particular case in Spain where a thorough prospection of local fruit tree species was performed in the 1950s with detailed data of the origin of each genotype but, unfortunately, the accessions are no longer conserved in ex situ germplasm collections. However, for most of those cultivars, an old stone collection is still preserved. In order to analyze the diversity present at the time when the prospection was made and to which extent variability has been eroded, we developed a protocol in apricot (Prunus armeniaca L.) to obtain DNA from maternal tissues of the stones of a sufficient quality to be amplified by PCR. The results obtained have been compared with the results from the profiles developed from apricot cultivars currently conserved in ex situ germplasm collections. The results highlight the fact that most of the old accessions are not conserved ex situ but provide a tool to prioritize the recovery of particular cultivars. The approach used in this work can also be applied to other plant species where seeds have been preserved.es_ES
dc.description.sponsorshipFinancial support for this work was provided by the Spanish Ministry of Science and Innovation – European Regional Development Fund (Project Grants AGL2010-15140, AGL2009-12621-CO2-01 and RF2008-00028-CO2-02), and Gobierno de Aragón (Grupo de Excelencia A-43). C.M. was supported by a grant from the Mediterranean Agronomic Institute of Zaragoza (IAMZ).es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's version-
dc.rightsopenAccesses_ES
dc.titleMolecular characterization of apricot germplasm from an old stone collectiones_ES
dc.typeartículoes_ES
dc.identifier.doi10.1371/journal.pone.0023979-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0023979es_ES
dc.identifier.e-issn1932-6203-
dc.identifier.pmid21901149-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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