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Molecular characterization of apricot germplasm from an old stone collection

AuthorsMartín, Carolina; Herrero Romero, María CSIC ORCID; Hormaza Urroz, José Ignacio
Issue DateAug-2011
PublisherPublic Library of Science
CitationMartín C, Herrero M, Hormaza JI. Molecular Characterization of Apricot Germplasm from an Old Stone Collection. Plos One 6 (8): e23979 (2011)
AbstractIncreasing germplasm erosion requires the recovery and conservation of traditional cultivars before they disappear. Here we present a particular case in Spain where a thorough prospection of local fruit tree species was performed in the 1950s with detailed data of the origin of each genotype but, unfortunately, the accessions are no longer conserved in ex situ germplasm collections. However, for most of those cultivars, an old stone collection is still preserved. In order to analyze the diversity present at the time when the prospection was made and to which extent variability has been eroded, we developed a protocol in apricot (Prunus armeniaca L.) to obtain DNA from maternal tissues of the stones of a sufficient quality to be amplified by PCR. The results obtained have been compared with the results from the profiles developed from apricot cultivars currently conserved in ex situ germplasm collections. The results highlight the fact that most of the old accessions are not conserved ex situ but provide a tool to prioritize the recovery of particular cultivars. The approach used in this work can also be applied to other plant species where seeds have been preserved.
Description9 Pag., 3 Tabl., 2 Fig. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher version (URL)http://dx.doi.org/10.1371/journal.pone.0023979
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