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Title

α4β1 integrin and 190-kDa CD44v constitute a cell surface docking complex for gelatinase B/MMP-9 in chronic leukemic but not in normal B cells

AuthorsRedondo-Muñoz, Javier ; Ugarte-Berzal, Estefanía ; García-Marco, José A.; Hernández del Cerro, Mercedes ; Van den Steen, Philippe E.; Opdenakker, Ghislain; Terol, María José; García-Pardo, Angeles
KeywordsNeoplasia
Leucemia linfocítica
Issue Date7-Mar-2008
PublisherAmerican Society of Hematology
CitationBlood, 112:169-178 (2008)
AbstractAs B-cell chronic lymphocytic leukemia (B-CLL) progresses, malignant cells extravasate and infiltrate lymphoid tissues. Several molecules, including gelatinase B/MMP-9, contribute to these processes. Although mainly a secreted protease, some MMP-9 is present at the B-CLL cell surface and the function, mode of anchoring, and interactions of this MMP-9 are unknown. Here we show that anti–MMP-9 antibodies immunoprecipitated a 190-kDa CD44v isoform and α4β1 integrin from B-CLL cells, but not from normal B cells. Function-blocking antibodies to α4β1 or CD44, or transfection with specific siRNAs, decreased cell-associated proMMP-9 and increased the secreted form. B-CLL cells attached to and bound proMMP-9 and active MMP-9, and this was inhibited by blocking the expression or function of α4β1 or CD44. The MMP-9 hemopexin domain was critical in these interactions. α4β1 and 190-kDa CD44v (but not CD44H) formed a complex at the cell surface, since they both coimmunoprecipitated with anti-α4, anti-β1, or anti-CD44 antibodies. Immunofluorescence analyses confirmed that α4β1 and CD44v colocalized with MMP-9. Binding of proMMP-9 inhibited B-CLL cell migration, and this required MMP-9 proteolytic activity. Thus, we have identified α4β1 and CD44v as a novel proMMP-9 cell surface docking complex and show that cell-associated MMP-9 may regulate B-CLL cell migration and arrest.
DescriptionBrief report.
Publisher version (URL)http://dx.doi.org/10.1182/blood-2007-08-109249
URIhttp://hdl.handle.net/10261/39607
DOIhttp://dx.doi.org/10.1182/blood-2007-08-109249
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