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http://hdl.handle.net/10261/3956
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Yruela Guerrero, Inmaculada | - |
dc.contributor.author | Torrado Plou, Elena | - |
dc.contributor.author | Roncel Gil, Mercedes | - |
dc.contributor.author | Picorel Castaño, Rafael | - |
dc.date.accessioned | 2008-04-30T14:10:51Z | - |
dc.date.available | 2008-04-30T14:10:51Z | - |
dc.date.issued | 2001-03 | - |
dc.identifier.citation | Photosynthesis Research 67(3): 199-206, 2001 | en_US |
dc.identifier.issn | 0166-8595 | - |
dc.identifier.uri | http://hdl.handle.net/10261/3956 | - |
dc.description | The definitive version is available at: http://www.springerlink.com/content/l304h18744r52202/fulltext.html | en_US |
dc.description.abstract | The light-induced difference absorption spectra associated to the photo-accumulation of reduced pheophytin a were studied in the isolated D1–D2–Cyt b559 complex in the presence of variable methyl viologen concentrations and different illumination conditions under anaerobiosis. Depending on the methyl viologen/reaction centre ratio, the relative intensities of the spectral bands at 681.5±0.5, 667.0±0.5 and 542.5±0.5 nm were modified. The reduced pheophytin a located at the D1-branch of the complex absorbs at 681.7±0.5 nm, and at least two additional pigment species contribute to the Qy band of the difference absorption spectra with maxima at 667.0±0.5 and 680.5±0.5 nm. We propose the additional species correspond to a peripheral chlorophyll a and the pheophytin a located at the D2-branch of the complex, respectively. The blue absorbing chlorophyll at 667 nm is susceptible to chemical redox changes with a midpoint reduction potential of +470 mV. The Qx absorption bands of both pheophytins localised at the D2- and D1-branch of the D1–D2–Cyt b559 complex were at 540.7±0.5 and 542.9±0.5, respectively. The results indicated that the two pheophytin molecules can be photoreduced in the D1–D2–Cyt b559 complex in certain experimental conditions. | en_US |
dc.description.sponsorship | This work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB98-1632). | en_US |
dc.format.extent | 321506 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | Springer Nature | en_US |
dc.rights | openAccess | en_US |
dc.subject | Absorption | en_US |
dc.subject | Chlorophyll | en_US |
dc.subject | Pheophytin | en_US |
dc.subject | Photosystem II | en_US |
dc.subject | Reaction centre | en_US |
dc.subject | Spectroscopy | en_US |
dc.title | Light-induced absorption spectra of the D1-D2-cytochrome b559 complex of the Photosystem II: Effect of methyl viologen concentration | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1023/A:1010682416016 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.identifier.e-issn | 1573-5079 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.fulltext | With Fulltext | - |
item.grantfulltext | open | - |
item.languageiso639-1 | en | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
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Yruela_Photosynth ResOct2000.pdf | 313,97 kB | Adobe PDF | Visualizar/Abrir |
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