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Título

Light-induced absorption spectra of the D1-D2-cytochrome b559 complex of the Photosystem II: Effect of methyl viologen concentration

AutorYruela Guerrero, Inmaculada ; Torrado Plou, Elena ; Roncel Gil, Mercedes ; Picorel Castaño, Rafael
Palabras claveAbsorption
Chlorophyll
Pheophytin
Photosystem II
Reaction centre
Spectroscopy
Fecha de publicaciónmar-2001
EditorSpringer
CitaciónPhotosynthesis Research 67(3): 199-206, 2001
ResumenThe light-induced difference absorption spectra associated to the photo-accumulation of reduced pheophytin a were studied in the isolated D1–D2–Cyt b559 complex in the presence of variable methyl viologen concentrations and different illumination conditions under anaerobiosis. Depending on the methyl viologen/reaction centre ratio, the relative intensities of the spectral bands at 681.5±0.5, 667.0±0.5 and 542.5±0.5 nm were modified. The reduced pheophytin a located at the D1-branch of the complex absorbs at 681.7±0.5 nm, and at least two additional pigment species contribute to the Qy band of the difference absorption spectra with maxima at 667.0±0.5 and 680.5±0.5 nm. We propose the additional species correspond to a peripheral chlorophyll a and the pheophytin a located at the D2-branch of the complex, respectively. The blue absorbing chlorophyll at 667 nm is susceptible to chemical redox changes with a midpoint reduction potential of +470 mV. The Qx absorption bands of both pheophytins localised at the D2- and D1-branch of the D1–D2–Cyt b559 complex were at 540.7±0.5 and 542.9±0.5, respectively. The results indicated that the two pheophytin molecules can be photoreduced in the D1–D2–Cyt b559 complex in certain experimental conditions.
DescripciónThe definitive version is available at: http://www.springerlink.com/content/l304h18744r52202/fulltext.html
URIhttp://hdl.handle.net/10261/3956
DOI10.1023/A:1010682416016
ISSN0166-8595 (print version)
1573-5079 (electronic version)
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