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Título: | Bacillus subtilis phage φ29 main promoters are efficiently recognized in vivo by the Streptomyces lividans RNA polymerase |
Autor: | Pulido, Diego CSIC ORCID; Jiménez Díaz, Antonio; Salas, Margarita CSIC ORCID ; Mellado, Rafael P. | Palabras clave: | Recombinant DNA Bacteriophage promoters S1 mapping Heterologous expression |
Fecha de publicación: | 1986 | Editor: | Elsevier | Citación: | Gene 49(3): 377-382 (1986) | Resumen: | A DNA fragment from the Bacillus subtilis phage φ29, containing the main early and late viral promoters, has been inserted upstream of the aminoglycoside phosphotransferase gene (neo) derived from the transposon Tn5 and present in a Streptomyces lividans promoter-probe plasmid. The φ29 promoters are specifically recognized by the S. lividans RNA polymerase which initiates transcription in vivo at the same sites utilized in B. subtilis. Moreover, the viral promoters efficiently direct the synthesis of high levels of the APHII enzyme in S. lividans. | Descripción: | Short communications | Versión del editor: | http://dx.doi.org/10.1016/0378-1119(86)90374-4 | URI: | http://hdl.handle.net/10261/39243 | DOI: | 10.1016/0378-1119(86)90374-4 | ISSN: | 0378-1119 |
Aparece en las colecciones: | (CBM) Artículos |
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