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Title

Observation of spermidine-induced attractive forces in self-assembled monolayers of single stranded DNA using a microcantilever sensor

AuthorsMertens, Johann ; Tamayo de Miguel, Francisco Javier ; Kosaka, Priscila M. ; Calleja, Montserrat
KeywordsBioMEMS
Cantilevers
Coatings
DNA
Gold
Micromechanical devices
Molecular biophysics
Monolayers
Self-assembly
Issue Date13-Apr-2011
PublisherAmerican Institute of Physics
CitationApplied Physics Letters 98(15): 153704 (2011)
AbstractDespite the biological relevance, the physical origin of attraction between highly negatively charged DNA strands in condensation remains an open question. We have used microcantilever sensors to study the forces involved in DNA condensation by spermidine. The experiments were performed under flow conditions with gold-coated cantilevers sensitized with thiolated single stranded DNA. The experiments show that above a critical concentration of spermidine, the DNA strands abruptly experiences large attractive forces. The critical spermidine concentration for the transition increases with the monovalent salt concentration. The experiments provide a direct insight of the forces responsible of condensation.
Description3 páginas, 3 figuras.
Publisher version (URL)http://dx.doi.org/10.1063/1.3580775
URIhttp://hdl.handle.net/10261/39102
DOI10.1063/1.3580775
ISSN0003-6951
Appears in Collections:(IMN-CNM) Artículos
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