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Influence of reduction in the elution times on HPLC glycohaemoglobin results

AuthorsLeón-Justel, Antonio; Santotoribio, José D.; Domínguez-Pascual, Inmaculada; Delgado, Ana L.; Macías, Carlos; Herrera, María T.; Leal Cerro, Alfonso ; Mangas, Miguel A.; Acosta, Domingo; Guerrero Montávez, Juan Miguel
Elution times
Issue Date16-Jul-2009
CitationClinical Biochemistry 42(15): 1582-1584 (2009)
AbstractObjectives To compare HPLC methods with short and long elution times for HbA1c measurement in blood. Methods Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). Results Passing–Bablok regression equations were: Y = 0.17 + 0.96X; Y = − 0.39 + 1.01X; Y = − 0.40 + 1.0X and the means of the differences using Bland–Altman Plot were 0.02; − 0.34; 0.32 for HA-8160/G7-Tosoh, HA-8160/Variant-II-Turbo and G7-Tosoh/Variant-II-Turbo, respectively. Conclusions Faster elution methods had no problems on reproducibility of results obtained by slower elution methods.
Description3 páginas, 1 tabla.
Publisher version (URL)http://dx.doi.org/10.1016/j.clinbiochem.2009.07.003
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