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dc.contributor.authorRosado, Aránzazu-
dc.contributor.authorZanella, Fabian-
dc.contributor.authorGarcía, Beatriz-
dc.contributor.authorCarnero, Amancio-
dc.contributor.authorLink, Wolfgang-
dc.identifier.citationPLoS ONE 3(3): e1823 (2008)es_ES
dc.description8 páginas, 5 figuras.es_ES
dc.description.abstractBackground Inhibition of Akt signaling is considered one of the most promising therapeutic strategies for many cancers. However, rational target-orientated approaches to cell based drug screens for anti-cancer agents have historically been compromised by the notorious absence of suitable control cells. Methodology/Principal Findings In order to address this fundamental problem, we have developed BaFiso, a live-cell screening platform to identify specific inhibitors of this pathway. BaFiso relies on the co-culture of isogenic cell lines that have been engineered to sustain interleukin-3 independent survival of the parental Ba/F3 cells, and that are individually tagged with different fluorescent proteins. Whilst in the first of these two lines cell survival in the absence of IL-3 is dependent on the expression of activated Akt, the cells expressing constitutively-activated Stat5 signaling display IL-3 independent growth and survival in an Akt-independent manner. Small molecules can then be screened in these lines to identify inhibitors that rescue IL-3 dependence. Conclusions/Significance BaFiso measures differential cell survival using multiparametric live cell imaging and permits selective inhibitors of Akt signaling to be identified. BaFiso is a platform technology suitable for the identification of small molecule inhibitors of IL-3 mediated survival signaling.es_ES
dc.description.sponsorshipThis work was supported by a grant from the Spanish MCyT (BIO2002-00197) and the Spanish MEC (project BIO2006-02432). F.Z. is the recipient of a Marie Curie Fellowship.es_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher’s version-
dc.subjectBase sequencees_ES
dc.subjectCoculture techniqueses_ES
dc.subjectDNA primerses_ES
dc.subjectProtein Kinase inhibitorses_ES
dc.subjectProto-oncogene proteins c-aktes_ES
dc.titleA Dual-Color Fluorescence-Based Platform to Identify Selective Inhibitors of Akt Signalinges_ES
dc.description.peerreviewedPeer reviewedes_ES
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