English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/3877
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Characterization of Genomic Clones and Expression Analysis of the Three Types of Superoxide Dismutases During Nodule Development in Lotus japonicus

AuthorsRubio Luna, María Carmen ; Becana Ausejo, Manuel ; Sato, Shusei; James, Euan Kevin; Tabata, Satoshi; Spaink, Herman P.
Keywordsdifferential gene expression
model legume
Issue DateMar-2007
PublisherAmerican Phytopathological Society
CitationMolecular Plant-Microbe Interactions, March 2007, Volume 20, Number 3, Pages 262-275
AbstractSuperoxide dismutases (SODs) are metalloenzymes that play a primary role in the protection against oxidative stress in plants and other organisms. We have characterized four SOD genes in Lotus japonicus and have analyzed their expression in roots and four developmental stages of nodules. The expression of cytosolic CuZnSOD, at the mRNA, protein, and enzyme activity levels, decreases with nodule age, and the protein is localized in the dividing cells and infection threads of emergent nodules and in the infected cells of young nodules. The mitochondrial MnSOD was downregulated, whereas the bacteroidal MnSOD displayed maximal protein and enzyme activity levels in older nodules. Two additional genes, encoding plastidic (FeSOD1) and cytosolic (FeSOD2) FeSOD isoforms, were identified and mapped. The genes are located in different chromosomes and show differential expression. The FeSOD1 mRNA level did not change during nodule development, whereas FeSOD2 was upregulated. The distinct expression patterns of the SOD genes may reflect different regulatory mechanisms of the enzyme activities during nodule ontogeny. In particular, at the mRNA and activity levels, the virtual loss of cytosolic CuZnSOD in mature and old nodules, concomitant with the induction of FeSOD2, suggests that the two enzymes may functionally compensate each other in the cytosol at the late stages of nodule development.
DescriptionThe definitive version is available at http://apsjournals.apsnet.org/loi/mpmi
URIhttp://hdl.handle.net/10261/3877
DOI10.1094/MPMI-20-3-0262
ISSN0894-0282
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
MPMI-20-3-0262.pdf969,27 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.