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Title

Development of a New High-Performance Liquid Chromatography–Electrospray Ionization Time-of-Flight Mass Spectrometry Method for the Determination of Low Molecular Mass Organic Acids in Plant Tissue Extracts

AuthorsRellán-Álvarez, Rubén ; López-Gomollón, Sara ; Abadía Bayona, Javier ; Álvarez-Fernández, Ana
Keywordscarboxylates
mass spectrometry;
organic acids
plant tissues
Issue DateJul-2011
PublisherAmerican Chemical Society
CitationRellan-Alvarez R, Lopez-Gomollon S, Abadia J, Alvarez-Fernandez A. Development of a New High-Performance Liquid Chromatography–Electrospray Ionization Time-of-Flight Mass Spectrometry Method for the Determination of Low Molecular Mass Organic Acids in Plant Tissue Extracts. Journal of agricultural and food chemistry 59 (13): 6864-6870 (2011)
AbstractA liquid chromatography–electrospray ionization time-of-flight mass spectrometry method has been developed for the direct and simultaneous determination of 10 low molecular mass organic acids in different plant tissue extracts. The method does not include a derivatization step. Quantification was accomplished using 13C-labeled malic and succinic acids as internal standards. Good limits of detection (0.05–1.27 pmol) were obtained for malic, 2-oxoglutaric, succinic, quinic, shikimic, cis-aconitic, and citric acids, whereas for oxalic, ascorbic, and fumaric acids limits of detection were 255, 25, and 11 pmol, respectively. Repeatability values for the retention time and peak area were <5%, with the exception of ascorbic acid. Analyte recovery was between 92% and 110% in most cases, with the exception of oxalic (39–108%), 2-oxoglutaric (44–69%), and ascorbic (22–86%) acids, which may require specific extraction procedures and use of the corresponding 13C-labeled organic acid as internal standards to improve accuracy. The method was applied to three types of plant materials: sugar beet leaf extracts, tomato xylem sap, and commercial orange juice.
Description7 Pag., 5 Tabl., 2 Fig.
Publisher version (URL)http://dx.doi.org/10.1021/jf200482a
URIhttp://hdl.handle.net/10261/38231
DOI10.1021/jf200482a
ISSN0021-8561
E-ISSN1520-5118
Appears in Collections:(EEAD) Artículos
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