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Title

Characterization of a 3'->5' exonuclease activity in the phage ø29-encoded DNA polymerase

AuthorsBlanco, Luis ; Salas, Margarita
Issue Date1985
PublisherOxford University Press
CitationNucleic Acids Research 13(4): 1239-1249 (1985)
AbstractPurified protein p2 of phage φ29, characterized as a specific DNA polymerase involved in the initiation and elongation of φ29 DNA replication, contains a 3′ → 5′ exonuclease active on single-stranded DNA, but not on double-stranded DNA. No 5′→3′ exonuclease activity was found. The 3′→5′ exonuclease activity was shown to be associated with the DNA polymerase since 1) the two activities were heat-inactivated with identical kinetics and 2) both activities, present in purified protein p2, cosedimented in a glycerol gradient.
Publisher version (URL)http://dx.doi.org/10.1093/nar/13.4.1239
URIhttp://hdl.handle.net/10261/38102
DOI10.1093/nar/13.4.1239
ISSN0305-1048
Appears in Collections:(CBM) Artículos
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