Suppressor-sensitive mutants of phage ø29

Abstract

Suppressor-sensitive mutants of phage Φ29 have been isolated and classified in 13 complementation groups. These mutants have been complemented with the temperature-sensitive mutants of Talavera et al. (1971) and mutant tsQ1360 reported in this paper; all together they make a total of 17 complementation groups. Two- and three-factor crosses have been carried out with sus and ts mutants, and 16 cistrons have been arranged in a linear genetic map. This map, integrating suppressor- and temperature-sensitive mutants, has a total of 24.2 recombination units.