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Title

Biophysical properties of bacteriophage ø29

AuthorsRubio, Víctor; Salas, Margarita CSIC ORCID ; Viñuela, Eladio; Usobiaga, P.; Saiz, José L.; Llopis, J. F.
Issue DateJan-1974
PublisherElsevier
CitationVirology 57(1): 112-121 (1974)
AbstractBacteriophage φ29 has been purified in large scale to homogeneity. The sedimentation and diffusion coefficients of the virus are (256 ± 2) × 10−13 sec and (8.8 ± 0.5) × 10−8 cm2 × sec−1, respectively. The value of the partial specific volume of φ29, obtained by pycnometry, is 0.61 ± 0.01 cm3 × g−1. From these values and the Svedberg equation, the molecular mass of φ29 is (18 ± 1) × 106 daltons. From the phosphorus and nitrogen content, and the amino acid and nucleotide composition, the DNA percentage in φ29 is 56 ± 2%. Other independent estimations of the molecular mass of φ29 are given.
Publisher version (URL)http://dx.doi.org/10.1016/0042-6822(74)90112-3
URIhttp://hdl.handle.net/10261/38020
DOI10.1016/0042-6822(74)90112-3
ISSN0042-6822
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