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Título

In vivo DNA binding of bacteriophage GA-1 protein p6

AutorAlcorlo, Martín; Salas, Margarita CSIC ORCID ; Hermoso, José Miguel
Palabras claveBacteriophage
Histone-like
Protein-DNA interaction
B. subtilis
X-ChIP
Fecha de publicación2007
EditorAmerican Society for Microbiology
CitaciónJournal of Bacteriology 189: 8024-8033 (2007)
ResumenBacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. In this work we show that it binds in vivo to the whole viral genome, as detected by means of cross-linking, chromatin immunoprecipitation and real-time PCR, having the characteristics of a histone-like protein. Binding to DNA of GA-1 protein p6 shows little supercoiling-dependency, in contrast with the ortholog protein of the evolutionary related Bacillus subtilis phage 29. This feature is a property of the protein rather than the DNA or the cellular background, since 29 protein p6 shows supercoiling-dependent binding to GA-1 DNA in Bacillus sp. strain G1R. GA-1 DNA replication is impaired in the presence of the gyrase inhibitors novobiocin and nalidixic acid, which indicates that, although non-covalently closed, the viral genome is topologically constrained in vivo. GA-1 protein p6 is also able to bind 29 DNA in B. subtilis cells; however, as expected, the binding is less supercoiling-dependent than the one observed with the 29 protein p6. In addition, the nucleoprotein complex formed is not functional, as it is not able to transcomplement the DNA replication deficiency of a 29 sus6 mutant. Furthermore, we took advantage of 29 protein p6 binding to GA-1 DNA to find that the viral DNA ejection mechanism seems to take place, as in the case of 29, with a right to left polarity in a two-step, push-pull process.
Versión del editorhttp://dx.doi.org/10.1128/JB.01047-07
URIhttp://hdl.handle.net/10261/37785
DOI10.1128/JB.01047-07
ISSN0021-9193
E-ISSN1098-5530
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