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A new plasmid vector for regulated gene expression in Bacillus subtilis

AuthorsSerrano-Heras, Gemma ; Salas, Margarita ; Bravo, Alicia
KeywordsBacillus subtilis
Plasmid vector
Regulated gene expression
Issue DateNov-2005
CitationPlasmid 54(3): 278-282 (2005)
AbstractWe have developed a novel regulated expression vector for Bacillus subtilis based on the Staphylococcus aureus plasmid pUB110. This vector, named pPR54, carries the PR promoter and the cI857 gene (encoding a temperature-sensitive transcriptional repressor) from the Escherichia coli phage λ. Using the gfp gene from the jellyfish Aequorea victoria as a reporter, we show that pPR54 is a useful vector for controllable production of heterologous proteins in B. subtilis.
Publisher version (URL)http://dx.doi.org/10.1016/j.plasmid.2005.04.002
Appears in Collections:(CBM) Artículos
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