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Comparative studies on lectin–carbohydrate interactions in low and high density homo- and heteroglycoclusters

AuthorsGómez García, Marta ; Benito, Juan M. ; Gutiérrez-Gallego, Ricardo; Maestre, Alfredo; Ortiz-Mellet, Carmen; García-Fernández, José Manuel; Jiménez Blanco, José L.
Surface Plasmon Resonance
Concanavalin A
Molecular sequence data
Issue Date24-Feb-2010
PublisherRoyal Society of Chemistry (UK)
CitationOrganic and Biomolecular Chemistry 8(8): 1849-1860 (2010)
AbstractA versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of “non-active” structural motifs in the presence of a certain density of “active” glycoligands.
Description12 páginas, 5 figuras, 3 tablas, 3 esquemas.
Publisher version (URL)http://dx.doi.org/10.1039/B920048G
Appears in Collections:(IIQ) Artículos
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