English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37484
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

The Cdc42 GEF Intersectin 2 controls mitotic spindle orientation to form the lumen during epithelial morphogenesis

AuthorsRodríguez-Fraticelli, Alejo E. ; Vergarajaúregui, Silvia ; Eastburn, Dennis J.; Datta, Anirban; Alonso, Miguel A. ; Mostov, Keith E.; Martín-Belmonte, Fernando
Keywordsdevelopment,
epithelial cells
GTPases
Cdc42
Issue Date17-May-2010
PublisherRockefeller University Press
CitationJournal of Cell Biology 189 (4): 725-738
AbstractEpithelial organs are made of tubes and cavities lined by a monolayer of polarized cells that enclose the central lumen. Lumen formation is a crucial step in the formation of epithelial organs. The Rho guanosine triphosphatase (GTPase) Cdc42, which is a master regulator of cell polarity, regulates the formation of the central lumen in epithelial morphogenesis. However, how Cdc42 is regulated during this process is still poorly understood. Guanine nucleotide exchange factors (GEFs) control the activation of small GTPases. Using the three-dimensional Madin–Darby canine kidney model, we have identified a Cdc42-specific GEF, Intersectin 2 (ITSN2), which localizes to the centrosomes and regulates Cdc42 activation during epithelial morphogenesis. Silencing of either Cdc42 or ITSN2 disrupts the correct orientation of the mitotic spindle and normal lumen formation, suggesting a direct relationship between these processes. Furthermore, we demonstrated this direct relationship using LGN, a component of the machinery for mitotic spindle positioning, whose disruption also results in lumen formation defects.
Publisher version (URL)http://dx.doi.org/10.1083/jcb.201002047
URIhttp://hdl.handle.net/10261/37484
DOI10.1083/jcb.201002047
ISSN0021-9258
Appears in Collections:(CBM) Artículos
Files in This Item:
File Description SizeFormat 
FMartinBelmonte_JCB_2010.pdf890,65 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.