English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/37306
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A role for tachykinins in the regulation of human sperm motility

AuthorsRavina, Cristina G.; Seda, Marian; Pinto Pérez, Francisco M. CSIC ORCID; Orea, Alicia CSIC; Fernández-Sánchez, Manuel; Pintado, C. Oscar; Candenas, M. Luz CSIC ORCID
KeywordsHuman sperm
mRNA expression
Tachykinin receptors
Issue Date16-Apr-2007
PublisherOxford University Press
CitationHuman Reproduction 22(6): 1617-1625 (2007)
AbstractBACKGROUND Tachykinins and tachykinin receptors are widely distributed in the male reproductive tract and appear to be involved in reproduction. However, the function and expression of tachykinins and their receptors in human spermatozoa remain poorly studied. We analysed the effects of tachykinins on sperm motility and characterized the population of tachykinin receptors in human spermatozoa. METHODS AND RESULTS Motility analysis was performed following World Health Organization guidelines and we found that substance P (SP), human hemokinin-1 (hHK-1), neurokinin A (NKA) and neurokinin B (NKB) produced concentration-dependent increases in sperm progressive motility. The effects of tachykinins were antagonized by the NK1 receptor-selective antagonist SR 140333, the NK2 receptor-selective antagonist, SR 48968 and, to a lesser extent, also by the NK3 receptor-selective antagonist SR 142801. Immunocytochemistry studies showed expression of the NK1, NK2 and NK3 tachykinin receptor proteins in spermatozoa with different major sites of localization for each receptor. Western blot analysis confirmed the presence of tachykinin receptors in sperm cell homogenates. RT–PCR demonstrated expression of the genes that encode SP/NKA (TAC1), NKB (TAC3) and hHK-1 (TAC4) but not the genes TACR1, TACR2 and TACR3 encoding NK1, NK2 and NK3 receptors, respectively. CONCLUSIONS These results show for the first time that the NK1, NK2 and NK3 tachykinin receptor proteins are present in human spermatozoa. Our findings suggest that tachykinins, probably acting through these three tachykinin receptors, play a role in the regulation of human sperm motility.
Description9 páginas, 6 figuras, 2 tablas.
Publisher version (URL)http://dx.doi.org/10.1093/humrep/dem069
Appears in Collections:(IIQ) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.