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Title

Disconnecting the Golgi ribbon from the centrosome prevents directional cell migration and ciliogenesis

AuthorsHurtado, Lidia; Caballero, Cristina; Gavilán, María P. ; Cárdenas, Jesús ; Bornens, Michel; Ríos, Rosa M.
KeywordsMammalian cells
Golgi ribbon
Issue Date23-May-2011
PublisherRockefeller University Press
CitationJournal of Cell Biology 193(5): 917-933 (2011)
AbstractMammalian cells exhibit a frequent pericentrosomal Golgi ribbon organization. In this paper, we show that two AKAP450 N-terminal fragments, both containing the Golgi-binding GM130-interacting domain of AKAP450, dissociated endogenous AKAP450 from the Golgi and inhibited microtubule (MT) nucleation at the Golgi without interfering with centrosomal activity. These two fragments had, however, strikingly different effects on both Golgi apparatus (GA) integrity and positioning, whereas the short fragment induced GA circularization and ribbon fragmentation, the large construct that encompasses an additional p150glued/MT-binding domain induced separation of the Golgi ribbon from the centrosome. These distinct phenotypes arose by specific interference of each fragment with either Golgi-dependent or centrosome-dependent stages of Golgi assembly. We could thus demonstrate that breaking the polarity axis by perturbing GA positioning has a more dramatic effect on directional cell migration than disrupting the Golgi ribbon. Both features, however, were required for ciliogenesis. We thus identified AKAP450 as a key determinant of pericentrosomal Golgi ribbon integrity, positioning, and function in mammalian cells.
DescriptionThis article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license.
Publisher version (URL)http://dx.doi.org/10.1083/jcb.201011014
URIhttp://hdl.handle.net/10261/36969
DOI10.1083/jcb.201011014
ISSN0021-9525
Appears in Collections:(CABIMER) Artículos
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