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Título : Molecular characterization of Miraflores peach variety and relatives using SSRs
Autor : Bouhadida, Mariem ; Casas Cendoya, Ana María ; Moreno Sánchez, María Ángeles ; Gogorcena Aoiz, Yolanda
Palabras clave : Clones
Genetic diversity
Genetic relationships
‘Miraflores’ peach variety
Traditional peach varieties
Fecha de publicación : 4-ene-2007
Editor: Elsevier
Citación : Scientia Horticulturae, Volume 111, Issue 2, 4 January 2007, Pages 140-145
Resumen: Some traditional peach varieties, originated from the region of Aragón (Spain), were analysed by SSRs (Simple Sequence Repeats). The aim of this research was to characterize 19 clones related to ‘Miraflores’ variety, with unknown pedigrees, to assess their genetic diversity and to elucidate their possible relationships with 10 traditional peach varieties. Twenty SSR primer pairs with high levels of polymorphism, which have been previously developed for peach, were used in this study. A total of 46 alleles were obtained for all the microsatellites studied, ranging from one to six alleles per locus, with a mean value of 2.3 alleles per locus. Fourteen SSRs were polymorphic in the set of varieties studied and permitted to distinguish 16 different genotypes out of the 30 initially studied, although fourteen ‘Miraflores’ clones showed identical gel profiles. The genetic distance matrix was used to construct Neighbor joining cluster and to perform principal coordinate analysis which allowed the arrangement of all the genotypes according to their genetic relationships. The genetic relationships among these traditional peach varieties, and in particular among ‘Miraflores’ clones are discussed. The obtained results confirm that microsatellite markers are very useful for these purposes.
Descripción : The definitive version is published in: http://www.sciencedirect.com/science/journal/03044238
URI : http://hdl.handle.net/10261/3673
DOI: 10.1016/j.scienta.2006.10.018
ISSN: 0304-4238
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