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Título: | Detergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre |
Autor: | Yruela Guerrero, Inmaculada CSIC ORCID ; García-Rubio, Inés CSIC ORCID; Roncel Gil, Mercedes CSIC ORCID CVN ; Martínez, Jesús I. CSIC ORCID; Ramiro Pascual, María Victoria CSIC; Ortega, José M. CSIC ORCID ; Alonso, Pablo J. CSIC ORCID; Picorel Castaño, Rafael CSIC ORCID | Palabras clave: | Cytochrome b559 Detergent Electron paramagnetic resonance photosystem II reaction centre redox potential |
Fecha de publicación: | 2003 | Editor: | Royal Society of Chemistry (UK) | Citación: | Photochem. Photobiol. Sci., 2003, (4),437-442 | Resumen: | The detergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1–D2–Cyt b559 complex preparations. Various n-dodecyl--D-maltoside concentrations from 0 to 0.2%(w/v) were used to stabilise the D1–D2–Cyt b559 complexes. Low spin heme EPR spectra were obtained but the gz feature positions changed depending on the detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b559 form (Em=+123–150 mV) in all the D1–D2–Cyt b559 complex preparations indicating that detergent does not affect this property of the protein in those conditions. A similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the protein. | Descripción: | The definitive version is available at: http://www.rsc.org/publishing/journals/PP/ | URI: | http://hdl.handle.net/10261/3630 | DOI: | 10.1039/b300187c |
Aparece en las colecciones: | (ICMA) Artículos (IBVF) Artículos (EEAD) Artículos |
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PPS03.01.03.pdf | 154,32 kB | Adobe PDF | Visualizar/Abrir |
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