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Detergent effect on cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre

AutorYruela Guerrero, Inmaculada ; García-Rubio, Inés; Roncel Gil, Mercedes ; Martínez, Jesús I.; Ramiro Pascual, María Victoria ; Ortega, José M. ; Alonso, Pablo J.; Picorel Castaño, Rafael
Palabras clavecytochrome b559
electron paramagnetic resonance
photosystem II
reaction centre
redox potential
Fecha de publicación2003
EditorRoyal Society of Chemistry (Great Britain)
CitaciónPhotochem. Photobiol. Sci., 2003, (4),437-442
ResumenThe detergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1–D2–Cyt b559 complex preparations. Various n-dodecyl--D-maltoside concentrations from 0 to 0.2%(w/v) were used to stabilise the D1–D2–Cyt b559 complexes. Low spin heme EPR spectra were obtained but the gz feature positions changed depending on the detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b559 form (Em=+123–150 mV) in all the D1–D2–Cyt b559 complex preparations indicating that detergent does not affect this property of the protein in those conditions. A similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the protein.
DescripciónThe definitive version is available at: http://www.rsc.org/publishing/journals/PP/
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