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dc.contributor.authorUgarte-Uribe, Begoña-
dc.contributor.authorPérez-Rentero, Sonia-
dc.contributor.authorLucas, Ricardo-
dc.contributor.authorAviñó, Anna-
dc.contributor.authorReina, José J.-
dc.contributor.authorAlkorta, Itziar-
dc.contributor.authorEritja Casadellà, Ramón-
dc.contributor.authorMorales, Juan C.-
dc.date.accessioned2011-05-27T10:07:51Z-
dc.date.available2011-05-27T10:07:51Z-
dc.date.issued2010-06-30-
dc.identifier.citationBioconjugate Chemistry 21(7): 1280-1287 (2010)es_ES
dc.identifier.issn1043-1802-
dc.identifier.urihttp://hdl.handle.net/10261/36165-
dc.description8 páginas, 5 figurases_ES
dc.description.abstractOligonucleotide conjugates carrying carbohydrates at the 5′-end have been prepared. Glucose, fucose, and saccharides containing glucose at the nonreducing end were attached to DNA strands using the classical phosphoramidite chemistry. Two types of spacers and a dendron scaffold helped to obtain a diversity of sugar presentations in the DNA conjugates. Cellular surface adsorption and cellular uptake of carbohydrate oligonucleotide antisense sequences were measured using flow cytometric analysis. Conjugates with the glucose moiety linked through long spacers (15 to 18 atom distances) were internalized better than those with short linkers (4 atom distance) and than DNA control strands without sugar modification. Conjugates with tetravalent presentation of glucose did not improve cell uptake.es_ES
dc.description.sponsorshipThis work was supported by Consejo Superior de Investigaciones Científicas (PIF06-045); Spanish Ministry of Science (grants BFU2007-63287, BFU2007-62062, CTQ2006-01123,CTQ2009-13705), Generalitat de Catalunya (2009/SGR/208),and Instituto de Salud Carlos III (CIBER-BNN, CB06_01_0019). RL thanks CSIC for a JAE contract.es_ES
dc.language.isoenges_ES
dc.publisherAmerican Chemical Societyes_ES
dc.rightsopenAccesses_ES
dc.subjectOligonucleotidees_ES
dc.subjectDNA conjugateses_ES
dc.subjectGlucosees_ES
dc.subjectCarbohydrateses_ES
dc.titleSynthesis, Cell-Surface Binding, and Cellular Uptake of Fluorescently Labeled Glucose−DNA Conjugates with Different Carbohydrate Presentationes_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1021/bc100079n-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1021/bc100079nes_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderGeneralitat de Catalunya-
dc.contributor.funderInstituto de Salud Carlos III-
dc.contributor.funderEuropean Commission-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002809es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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