Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/3614
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dc.contributor.authorDaròs Arnau, José Antonio-
dc.contributor.authorFlores, Ricardo-
dc.date.accessioned2008-04-16T09:39:09Z-
dc.date.available2008-04-16T09:39:09Z-
dc.date.issued2002-
dc.identifier.citationEMBO Journal 21(4): 749–759 (2002)en_US
dc.identifier.issn1460-2075-
dc.identifier.urihttp://hdl.handle.net/10261/3614-
dc.descriptionFinal full-text version of the paper available at http://www.nature.com/emboj/index.html-
dc.description.abstractViroids, small single-stranded circular RNAs (246–401 nucleotides), do not have mRNA capacity and must recruit host proteins to assist in the steps of their biological cycle. The nature of these cellular factors is poorly understood due to a lack of reliable experimental approaches. Here, to screen for host proteins interacting with viroid RNAs in vivo, we UV-irradiated avocado leaves infected with avocado sunblotch viroid (ASBVd), the type member of chloroplast viroids containing hammerhead ribozymes. This resulted in the detection of several ASBVd–host protein adducts. Tandem mass spectrometry analysis of the most abundant cross-linked species identified the protein component as two closely related chloroplast RNA-binding proteins (PARBP33 and PARBP35) of a family whose members previously have been shown to be involved in stabilization, maturation and editing of chloroplast transcripts. PARBP33 behaves as an RNA chaperone that stimulates in vitro the hammerhead-mediated self-cleavage of the multimeric ASBVd transcripts that result from rolling circle replication, indicating that this reaction, despite its RNA-based mechanism, is facilitated by proteins. The structural and functional parallelism between PARBP33 and PARBP35, and some proteins involved in viral RNA replication, indicates that viroids and RNA viruses recruit similar host proteins for their replication.en_US
dc.description.sponsorshipR.F. was partially supported by grant PB98-0500 of the DGES de España. J.A.D. was the recipient of a postdoctoral contract from the Ministerio de Ciencia y Tecnología de España.en_US
dc.format.extent350274 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.rightsclosedAccessen_US
dc.subjectAvocado sunblotch viroiden_US
dc.subjectCatalytic RNAsen_US
dc.subjectHammerhead ribozymesen_US
dc.subjectRNA-binding proteinsen_US
dc.subjectRNA chaperonesen_US
dc.titleA chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavageen_US
dc.typeartículoen_US
dc.identifier.doi10.1093/emboj/21.4.749-
dc.description.peerreviewedPeer revieweden_US
dc.identifier.pmid11847122-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
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