English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/36125
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Neurogenin 3 cellular and subcellular localization in the developing and adult hippocampus

AuthorsSimón-Areces, Julia; Membrive, Gema; García Fernández, Carmen; García-Segura, Luis M. ; Arévalo, María Ángeles
Keywordsproneural gene
neuron development
pyramidal layer
dentate gyrus
Issue Date2010
PublisherJohn Wiley & Sons
CitationThe Journal of Comparative Neurology 518 (10):1814–1824 (2010)
AbstractNeurogenin 3 (Ngn3), a proneural gene controlled by the Notch receptor, is implicated in the control of dendrite morphology and synaptic plasticity of cultured hippocampal neurons. Here we report the localization and subcellular distribution of Ngn3 in the hippocampus in vivo and in neuronal cultures. In situ hybridization showed Ngn3 mRNA expression in the pyramidal layer and dentate gyrus of adult mouse hippocampus. Immunohistochemistry studies revealed that Ngn3 localization is mostly cytoplasmic in the hippocampal eminence at embryonic day (E)17 and postnatal day (P)0. At P10 it is cytoplasmic in CA1–CA3 pyramidal neurons and nuclear in granule cells of the dentate gyrus. In the adult hippocampus Ngn3 is localized in the nucleus and cytoplasm of both pyramidal neurons and granule cells. During development of cultured hippocampal neurons, Ngn3 mRNA expression is higher at stages of neuronal polarization, as judged by reverse-transcription polymerase chain reaction (RT-PCR), and it is mostly cytoplasmic. The tracking of the subcellular localization of Ngn3 in neurons infected with a virus expressing myc-Ngn3 suggests that the protein is quickly translocated to the cell nucleus after synthesis and then reexported to the cytoplasm. Treatment with leptomycinB, a potent and specific inhibitor of the exportin CRM1, induced its accumulation into the nucleus, suggesting that CRM1 mediates the nuclear export of Ngn3. These results suggest that Ngn3 may play a role in neuronal development by actions in the cytoplasm.
Description11 pages, 3 figures. - PMID: 20235092 [PubMed - indexed for MEDLINE]
Publisher version (URL)http://onlinelibrary.wiley.com/doi/10.1002/cne.22304/abstract
URIhttp://hdl.handle.net/10261/36125
DOI10.1002/cne.22304
ISSN0021-9967
E-ISSN1550-7130
Appears in Collections:(IC) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.