Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/3575
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Fluidization of Membrane Lipids Enhances the Tolerance of Saccharomyces cerevisiae to Freezing and Salt Stress

AuthorsRodríguez Vargas, Sonia CSIC; Sánchez-García, Alicia CSIC ORCID; Martínez-Rivas, José Manuel CSIC ORCID ; Prieto Alamán, José Antonio CSIC ORCID; Rández Gil, Francisca CSIC ORCID
Issue DateJan-2007
PublisherAmerican Society for Microbiology
CitationApplied and Environmental Microbiology 73(1): 110–116 (2007)
AbstractUnsaturated fatty acids play an essential role in the biophysical characteristics of cell membranes and determine the proper function of membrane-attached proteins. Thus, the ability of cells to alter the degree of unsaturation in their membranes is an important factor in cellular acclimatization to environmental conditions. Many eukaryotic organisms can synthesize dienoic fatty acids, but Saccharomyces cerevisiae can introduce only a single double bond at the Δ9 position. We expressed two sunflower (Helianthus annuus) oleate Δ12 desaturases encoded by FAD2-1 and FAD2-3 in yeast cells of the wild-type W303-1A strain (trp1) and analyzed their effects on growth and stress tolerance. Production of the heterologous desaturases increased the content of dienoic fatty acids, especially 18:2Δ9,12, the unsaturation index, and the fluidity of the yeast membrane. The total fatty acid content remained constant, and the level of monounsaturated fatty acids decreased. Growth at 15°C was reduced in the FAD2 strains, probably due to tryptophan auxotrophy, since the trp1 (TRP1) transformants that produced the sunflower desaturases grew as well as the control strain did. Our results suggest that changes in the fluidity of the lipid bilayer affect tryptophan uptake and/or the correct targeting of tryptophan transporters. The expression of the sunflower desaturases, in either Trp+ or Trp− strains, increased NaCl tolerance. Production of dienoic fatty acids increased the tolerance to freezing of wild-type cells preincubated at 30°C or 15°C. Thus, membrane fluidity is an essential determinant of stress resistance in S. cerevisiae, and engineering of membrane lipids has the potential to be a useful tool of increasing the tolerance to freezing in industrial strains.
Publisher version (URL)http://dx.doi.org/10.1128/AEM.01360-06
URIhttp://hdl.handle.net/10261/3575
DOI10.1128/AEM.01360-06
ISSN1098-5336
Appears in Collections:(IATA) Artículos
(IG) Artículos

Files in This Item:
File Description SizeFormat
AEM2007-Rodriguez.pdf1,32 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

PubMed Central
Citations

43
checked on May 22, 2022

SCOPUSTM   
Citations

148
checked on May 18, 2022

WEB OF SCIENCETM
Citations

136
checked on May 21, 2022

Page view(s)

399
checked on May 22, 2022

Download(s)

259
checked on May 22, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.