English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3575
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 80 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Fluidization of Membrane Lipids Enhances the Tolerance of Saccharomyces cerevisiae to Freezing and Salt Stress
Autor : Rodríguez Vargas, Sonia ; Sánchez-García, Alicia ; Martínez-Rivas, José Manuel ; Prieto Alamán, José Antonio ; Rández Gil, Francisca
Fecha de publicación : ene-2007
Editor: American Society for Microbiology
Citación : Applied and Environmental Microbiology 73(1): 110–116 (2007)
Resumen: Unsaturated fatty acids play an essential role in the biophysical characteristics of cell membranes and determine the proper function of membrane-attached proteins. Thus, the ability of cells to alter the degree of unsaturation in their membranes is an important factor in cellular acclimatization to environmental conditions. Many eukaryotic organisms can synthesize dienoic fatty acids, but Saccharomyces cerevisiae can introduce only a single double bond at the Δ9 position. We expressed two sunflower (Helianthus annuus) oleate Δ12 desaturases encoded by FAD2-1 and FAD2-3 in yeast cells of the wild-type W303-1A strain (trp1) and analyzed their effects on growth and stress tolerance. Production of the heterologous desaturases increased the content of dienoic fatty acids, especially 18:2Δ9,12, the unsaturation index, and the fluidity of the yeast membrane. The total fatty acid content remained constant, and the level of monounsaturated fatty acids decreased. Growth at 15°C was reduced in the FAD2 strains, probably due to tryptophan auxotrophy, since the trp1 (TRP1) transformants that produced the sunflower desaturases grew as well as the control strain did. Our results suggest that changes in the fluidity of the lipid bilayer affect tryptophan uptake and/or the correct targeting of tryptophan transporters. The expression of the sunflower desaturases, in either Trp+ or Trp− strains, increased NaCl tolerance. Production of dienoic fatty acids increased the tolerance to freezing of wild-type cells preincubated at 30°C or 15°C. Thus, membrane fluidity is an essential determinant of stress resistance in S. cerevisiae, and engineering of membrane lipids has the potential to be a useful tool of increasing the tolerance to freezing in industrial strains.
Versión del editor: http://dx.doi.org/10.1128/AEM.01360-06
URI : http://hdl.handle.net/10261/3575
DOI: 10.1128/AEM.01360-06
ISSN: 1098-5336
Aparece en las colecciones: (IATA) Artículos
(IG) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
AEM2007-Rodriguez.pdf1,32 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.