English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3563
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase
Autor : Romero, Francisco; Multon, Marie-Christine; Ramos-Morales, Francisco; Domínguez, África; Bernal, Juan A.; Pintor-Toro, José Antonio ; Tortolero, María
Fecha de publicación : 2001
Editor: Oxford University Press
Citación : Nucleic Acids Research 29(6): 1300–1307 (2001)
Resumen: We have previously isolated the hpttg proto-oncogene, which is expressed in normal tissues containing proliferating cells and in several kinds of tumors. In fact, expression of hPTTG correlates with cell proliferation in a cell cycle-dependent manner. Recently it was reported that PTTG is a vertebrate analog of the yeast securins Pds1 and Cut2, which are involved in sister chromatid separation. Here we show that hPTTG binds to Ku, the regulatory subunit of the DNA-dependent protein kinase (DNA-PK). hPTTG and Ku associate both in vitro and in vivo and the DNA-PK catalytic subunit phosphorylates hPTTG in vitro. Furthermore, DNA double-strand breaks prevent hPTTG–Ku association and disrupt the hPTTG–Ku complexes, indicating that genome damaging events, which result in the induction of pathways that activate DNA repair mechanisms and halt cell cycle progression, might inhibit hPTTG–Ku interaction in vivo. We propose that hPTTG might connect DNA damage-response pathways with sister chromatid separation, delaying the onset of mitosis while DNA repair occurs.
Descripción : 8 pages, 5 figures, 58 references.
URI : http://hdl.handle.net/10261/3563
ISSN: 1362-4962
Aparece en las colecciones: (IRNAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Human securin, hPTTG, is associated with Ku heterodimer.pdf263,97 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.