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Título : Hydrogen-exchange stability analysis of Bergerac-Src homology 3 variants allows the characterization of a folding intermediate in equilibrium
Autor : Viguera, Ana Rosa; Serrano, Luis
Palabras clave : Kinetics
β-hairpin
Fecha de publicación : may-2003
Editor: National Academy of Sciences (U.S.)
Citación : Proceedings of the National Academy of Sciences 100(10): 5730–5735 (2003)
Resumen: Amide hydrogen/deuterium exchange rates have been determined for two mutants of α-spectrin Src homology 3 domain (WT), containing an elongated stable (SHH) and unstable (SHA) distal loop. SHA, similarly to WT, follows a two-state transition, whereas SHH apparently folds via a three-state mechanism. Native-state amide hydrogen exchange is effective in ascribing energetic readjustments observed in kinetic experiments to species stabilized within the denatured base and distinguishing those from high-energy barrier crossings. Comparison of ΔGex and mex parameters for amide protons of these mutants demonstrates the existence of an intermediate and allows the identification of protons protected in this state. The consolidation of a form containing a prefolded long β-hairpin induces the switch to a three-state mechanism in an otherwise two-state folder. It can be inferred that the unbalanced high stability of individual elements of secondary structure in a polypeptide could ultimately complicate its folding mechanism.
Descripción : Copyright © by National Academy of Sciences. Final full-text version of the paper available at: http://www.pnas.org/content/vol100/issue10/
URI : http://hdl.handle.net/10261/3558
DOI: 10.1073/pnas.0837456100
ISSN: 1091-6490
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