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Optimization of Bacteriocin Production by Batch Fermentation of Lactobacillus plantarum LPCO10

AutorLeal-Sánchez, M. Vega; Jiménez Díaz, Rufino ; Maldonado-Barragán, Antonio ; Garrido Fernández, A. ; Ruiz-Barba, José Luis
Fecha de publicaciónsep-2002
EditorAmerican Society for Microbiology
CitaciónApplied and Environmental Microbiology 68(9): 4465–4471 (2002)
ResumenOptimization of bacteriocin production by Lactobacillus plantarum LPCO10 was explored by an integral statistical approach. In a prospective series of experiments, glucose and NaCl concentrations in the culture medium, inoculum size, aeration of the culture, and growth temperature were statistically combined using an experimental 23 5-2 fractional factorial two-level design and tested for their influence on maximal bacteriocin production by L. plantarum LPCO10. After the values for the less-influential variables were fixed, NaCl concentration, inoculum size, and temperature were selected to study their optimal relationship for maximal bacteriocin production. This was achieved by a new experimental 32 3-1 fractional factorial three-level design which was subsequently used to build response surfaces and analyzed for both linear and quadratic effects. Results obtained indicated that the best conditions for bacteriocin production were shown with temperatures ranging from 22 to 27°C, salt concentration from 2.3 to 2.5%, and L. plantarum LPCO10 inoculum size ranging from 107.3 to 107.4 CFU/ml, fixing the initial glucose concentration at 2%, with no aeration of the culture. Under these optimal conditions, about 3.2 104 times more bacteriocin per liter of culture medium was obtained than that used to initially purify plantaricin S from L. plantarum LPCO10 to homogeneity. These results indicated the importance of this study in obtaining maximal production of bacteriocins from L. plantarum LPCO10 so that bacteriocins can be used as preservatives in canned foods.
URIhttp://hdl.handle.net/10261/3532
DOI10.1128/AEM.68.9.4465-4471.2002
ISSN1098-5336
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