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http://hdl.handle.net/10261/35189
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Title: | Development of a cost-effective pyrosequencing approach for SNP genotyping in barley |
Authors: | Silvar Casao, Cristina ![]() ![]() ![]() |
Keywords: | SNP pyrosequencing M13 tail barley breeding marker-assisted selection cost-effective |
Issue Date: | Jun-2011 |
Publisher: | John Wiley & Sons |
Citation: | Silvar C, Perovic D, Casas AM, Igartua E, Ordon F. Development of a cost-effective pyrosequencing approach for SNP genotyping in barley. Plant breeding 130 (3): 394-397 (2011) |
Abstract: | An improved, efficient, reliable and cost-effective pyrosequencing protocol for single nucleotide polymorphism (SNP) genotyping in plants is described. Labelling of the PCR products, required for the single-stranded pyrosequencing assay, is carried out in a one-step PCR with a universal biotinalyted M13 primer of 19-bp. A ratio of 1 : 10 of tailed primer to M13 primer in a 15-μl reaction volume turned out to be best suited for a successful identification of different alleles based on known SNPs. This technology allows cost-effective SNP genotyping in plant genomes for loci of interest at an overall low cost in a short period of time and is therefore best suited for marker-based selection procedures. |
Description: | 12 Pag., 1 Tabl., 1 Fig. The definitive version is available at: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1439-0523 |
Publisher version (URL): | http://dx.doi.org/10.1111/j.1439-0523.2011.01853.x |
URI: | http://hdl.handle.net/10261/35189 |
DOI: | 10.1111/j.1439-0523.2011.01853.x |
ISSN: | 0179-9541 |
E-ISSN: | 1439-0523 |
Appears in Collections: | (EEAD) Artículos |
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SilvarC_Plant Breeding_2011b.pdf | 264,47 kB | Adobe PDF | ![]() View/Open |
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