English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3510
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

The RpoT Regulon of Pseudomonas putida DOT-T1E and Its Role in Stress Endurance against Solvents

AutorDuque, Estrella; Rodríguez-Herva, José J.; Torre, Jesús de la; Domínguez-Cuevas, Patricia; Muñoz-Rojas, Jesús; Ramos, Juan L.
Fecha de publicaciónene-2007
EditorAmerican Society for Microbiology
CitaciónJournal of Bacteriology 189(1): 207–219 (2007)
ResumenPseudomonas putida encodes 20 extracytoplasmic sigma factors (ECFs). In this study, we show that one of these ECFs, known as ECF-Pp12 (PP3006), plays a role in tolerance of toluene and other organic solvents. Based on this finding, we have called the gene that encodes this new ECF rpoT. The rpoT gene forms an operon with the preceding gene and with the gene located downstream. The translated gene product of the open reading frame PP3005 is an inner membrane protein, whereas the PP3007 protein is periplasmic. A nonpolar rpoT mutant was generated by homologous recombination, and survival of the mutant was tested under various stress conditions. The mutant strain was hypersensitive to toluene and other solvents but just as tolerant as the wild type of stress imposed by heat, antibiotics, NaCl, paraquat, sodium dodecyl sulfate, H2O2, and benzoate. In the rpoT mutant background, expression of around 50 transcriptional units was affected: 31 cistrons were upregulated, and 23 cistrons were downregulated. This indicates that about 1% of all P. putida genes are under the direct or indirect influence of RpoT. The rpoT gene controls the expression of a number of membrane proteins, including components of the respiratory chains, porins, transporters, and multidrug efflux pumps. Hypersensitivity of the P. putida RpoT-deficient mutant to organic solvents can be attributed to the fact that in the rpoT strain, expression of the toluene efflux pump ttgGHI genes is severalfold lower than in the parental strain.
Versión del editorhttp://dx.doi.org/10.1128/JB.00950-06
URIhttp://hdl.handle.net/10261/3510
DOI10.1128/JB.00950-06
ISSN1098-5530
Aparece en las colecciones: (EEZ) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.