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Título : Identification of the Initial Steps in d-Lysine Catabolism in Pseudomonas putida
Autor : Revelles, Olga; Wittich, Rolf-Michael; Ramos, Juan L.
Fecha de publicación : abr-2007
Editor: American Society for Microbiology
Citación : Journal of Bacteriology 189(7): 2787–2792 (2007)
Resumen: Pseudomonas putida uses L-lysine as the sole carbon and nitrogen source which preferentially requires its metabolism through two parallel pathways. In one of the pathways -aminovalerate is the key metabolite, whereas in the other L-lysine is racemized to D-lysine, and L-pipecolate and -aminoadipate are the key metabolites. All the genes and enzymes involved in the D-lysine pathway, except for those involved in the conversion of D-lysine into 1-piperideine-2-carboxylate, have been identified previously (30). In this study we report that the conversion of D-lysine into 1-piperideine-2-carboxylate can be mediated by a D-lysine aminotransferase (PP3590) and a D-lysine dehydrogenase (PP3596). From a physiological point of view PP3596 plays a major role in the catabolism of D-lysine since its inactivation leads to a marked reduction in the growth rate with L- or D-lysine as the sole carbon and nitrogen source, whereas inactivation of PP3590 leads only to slowed growth. The gene encoding PP3590, called here amaC, forms an operon with dpkA, the gene encoding the enzyme involved in conversion of 1-piperideine-2-carboxylate to L-pipecolate in the D-lysine catabolic pathway. The gene encoding PP3596, called here amaD, is the fifth gene in an operon made up of seven open reading frames (ORFs) encoding PP3592 through PP3597. The dpkA amaC operon was transcribed divergently from the operon ORF3592 to ORF3597. Both promoters were mapped by primer extension analysis, which showed that the divergent 35 hexamers of these operon promoters were adjacent to each other. Transcription of both operons was induced in response to L- or D-lysine in the culture medium.
Versión del editor: http://dx.doi.org/10.1128/JB.01538-06
URI : http://hdl.handle.net/10261/3508
DOI: 10.1128/JB.01538-06
ISSN: 1098-5530
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