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Title

Structural properties of g,t-parallel duplexes

AuthorsAviñó, Anna CSIC ORCID; Cubero, Elena; Gargallo, Raimundo; González, Carlos CSIC ; Orozco, Modesto; Eritja Casadellà, Ramón CSIC ORCID
Issue Date21-Feb-2010
PublisherHindawi Publishing Corporation
CitationJournal of Nucleic Acids 2010: 763658 (2010)
AbstractThe structure of G,T-parallel-stranded duplexes of DNA carrying similar amounts of adenine and guanine residues is studied by means of molecular dynamics (MD) simulations and UV- and CD spectroscopies. In addition the impact of the substitution of adenine by 8-aminoadenine and guanine by 8-aminoguanine is analyzed. The presence of 8-aminoadenine and 8-aminoguanine stabilizes the parallel duplex structure. Binding of these oligonucleotides to their target polypyrimidine sequences to form the corresponding G,T-parallel triplex was not observed. Instead, when unmodified parallel-stranded duplexes were mixed with their polypyrimidine target, an interstrand Watson-Crick duplex was formed. As predicted by theoretical calculations parallel-stranded duplexes carrying 8-aminopurines did not bind to their target. The preference for the parallel-duplex over the Watson-Crick antiparallel duplex is attributed to the strong stabilization of the parallel duplex produced by the 8-aminopurines. Theoretical studies show that the isomorphism of the triads is crucial for the stability of the parallel triplex.
Description11 pages, 8 figures, 6 tables.-- PMID: 20798879[PubMed].-- PMCID: PMC2925217.-- Supplemental information available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2925217/bin/763658.f1.pdf
Publisher version (URL)http://dx.doi.org/10.4061/2010/763658
URIhttp://hdl.handle.net/10261/35019
DOI10.4061/2010/763658
ISSN2090-0201
E-ISSN2090-021X
Appears in Collections:(IQAC) Artículos

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