English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/3486
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 15 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar EndNote (RIS)Exportar EndNote (RIS)
Título : Characterization of the human DYRK1A promoter and its regulation by the transcription factor E2F1
Autor : Maenz, Barbara; Hekerman, Paul; Vela, Eva M.; Galcerán, Juan; Becker, Walter
Fecha de publicación : 26-mar-2008
Editor: BioMed Central
Citación : BMC Molecular Biology 9: 30 (2008)
Resumen: [Background] Overexpression of the human DYRK1A gene due to the presence of a third gene copy in trisomy 21 is thought to play a role in the pathogenesis of Down syndrome. The observation of gene dosage effects in transgenic mouse models implies that subtle changes in expression levels can affect the correct function of the DYRK1A gene product. We have therefore characterized the promoter of the human DYRK1A gene in order to study its transcriptional regulation
[Results] Transcription start sites of the human DYRK1A gene are distributed over 800 bp within a region previously identified as an unmethylated CpG island. We have identified a new alternative noncoding 5'-exon of the DYRK1A gene which is located 772 bp upstream of the previously described transcription start site. Transcription of the two splicing variants is controlled by non-overlapping promoter regions that can independently drive reporter gene expression. We found no evidence of cell- or tissue-specific promoter usage, but the two promoter regions differed in their activity and their regulation. The sequence upstream of exon 1A (promoter region A) induced about 10-fold higher reporter gene activity than the sequence upstream of exon 1B (promoter region B). Overexpression of the transcription factor E2F1 increased DYRK1A mRNA levels in Saos2 and Phoenix cells and enhanced the activity of promoter region B three- to fourfold.
[Conclusions] The identification of two alternatively spliced transcripts whose transcription is initiated from differentially regulated promoters regions indicates that the expression of the DYRK1A gene is subject to complex control mechanisms. The regulatory effect of E2F1 suggests that DYRK1A may play a role in cell cycle regulation or apoptosis.
Descripción : Contiene además dos ficheros adicionales: Supplementary figures y Vector construction and oligonucleotide sequences.
Versión del editor: http://dx.doi.org/10.1186/1471-2199-9-30
URI : http://hdl.handle.net/10261/3486
DOI: 10.1186/1471-2199-9-30
ISSN: 1471-2199
Aparece en las colecciones: (IN) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
supp1.pdfArchivo adicional 11,79 MBAdobe PDFVista previa
Visualizar/Abrir
supp2.pdfArchivo adicional 133,88 kBAdobe PDFVista previa
Visualizar/Abrir
human.pdfPrincipal745,16 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.