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p-Chlorophenylalanine increases tryptophan hydroxylase mRNA levels in the raphe nuclei. A time course study using in situ hybridization.

AuthorsCortés, Roser ; Mengod Los Arcos, Guadalupe ; Celada, Pau ; Artigas, Francesc
Serotonin system
Dorsal raphe
Gene regulation
In situ hybridization
Issue DateFeb-1993
PublisherJohn Wiley & Sons
CitationJournal of Neurochemistry 60 (2) : 761-764 (1993)
AbstractThe effects of a single dose of p-chlorophenylalanine on the mRNA encoding tryptophan-5-hydroxylase (EC in the rat dorsal raphe nucleus were analyzed using in situ hybridization. The levels of tryptophan-5-hydroxylase mRNA were markedly increased in cell bodies located in the ventromedial part of the dorsal raphe 1–2 days after p-chlorophenylalanine (300 mg/kg, i.p.) administration. This was followed by a decrease in the amount of tryptophan-5-hydroxylase mRNA, which returned to basal values by 5 days after treatment. An almost symmetric time course was observed for the midbrain serotonin concentration. Our results on the temporal pattern of changes in tryptophan-5-hydroxylase mRNA levels in the ventromedial part of the dorsal raphe are opposite to those reported for the enzyme activity and serotonin concentration after p-chlorophenylalanine treatment. These changes may result from modifications in enzyme mRNA expression, suggesting that tryptophan-5-hydroxylase gene transcription is involved in feedback mechanisms regulating serotonin synthesis.
Publisher version (URL)http://dx.doi.org/10.1111/j.1471-4159.1993.tb03213.x
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